Table 1 rtPCR primer sets for gene expression measurements
From: EGR1 controls divergent cellular responses of distinctive nucleus pulposus cell types
Symbol | Forward primer | Reverse primer |
|---|---|---|
ADAMTS4 | GGTCATGTCTTCAACATGCTCC | AGGATCCACATGAGCCATCAC |
COL1A1 | TGGAGAGTACTGGATTGACCCC | TGCAGAAGACTTTGATGGCATC |
COL2A1 | TGGGTGTTCTATTTATTTATTGTCTTCCT | GCGTTGGACTCACACCAGTTAGT |
COX2 | ACCAACATGATGTTTGCATTCTTT | GGTCCCCGCTTAAGATCTGTCT |
EGR1 | TGACCGCAGAGTCTTTTCCT | TGGGTTGGTCATGCTCACTA |
KRT19 | GCAGTCACAGCTGAGCATGAA | TCCGTTTCTGCCAGTGTGTCT |
MMP3 | TGATGAACAATGGACAAAGGATACA | TTTCATGAGCAGCAACGAGAA |
SOX9 | AGTACCCGCACCTGCACAAC | CGCTTCTCGCTCTCGTTCAG |
T | CCACCTGCAAATCCTCATCCT | TTGGAGAATTGTTCCGATGAG |
TNFα | TCAATCGGCCCGACTATCTC | CAGGGCAATGATCCCAAAGT |
VCAN | TCCCTCACTGTGGTCAAG | GTGTGTACCTGCTGGTTG |
βACT | CCTGGCACCCAGCACAAT | GCCGATCCACACGGAGTACT |
CYCLO | CCTGCTTCCACCGGATCAT | CGTTGTGGCGCGTAAAGTC |
EIF2B1 | TGTCAGGTAAGAAAATGGCCAAA | TGTAGCCGACAGCAGCATCT |
MRPL19 | CGCCGAAACCGGTCATC | TCCCCTTCGAGGAATGAATTC |