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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: EGR1 controls divergent cellular responses of distinctive nucleus pulposus cell types

Fig. 3

Confirmation loss of EGR1 function. a NP-nR and NP-R clones were transfected with 30 nM siRNAs targeting EGR1 (E) or control (C) siRNA. Sixteen hours after transfection cells were harvested (time point t = 0) and stimulated with IL-1β for 2 and 48 h. Immunoblotting for EGR1 reveals efficient knock-down of EGR1 at two hours post stimulation in both cell lines. Βeta Actin (βACT) was used as loading control. b Basal expression of COX2, TNFα, MMP3, and ADAMTS4 genes implicated in disc degeneration at t0. Gene expression was normalized to Cyclophillin B; asterisks indicate significantly different expression level; p < 0.05. c In silico representation of the promotor region of genes implicated in disc degeneration (Genomatix software; http://www.genomatix.de). Putative transcription factor (TF) binding sites for EGR family members are depicted by yellow boxes; high-probability EGR binding sites are indicated by black/bold print arrow heads, low-probability binding sites by light grey/normal print. Black arrows indicate the transcription start site

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