Fig. 8

Histology and immunohistochemistry of cartilage. Full thickness articular cartilage was used to prepare frozen sections. Aggrecan in the sections was visualized either by staining with Safranin O or by exposure to antibodies recognizing the G1 region (αG1) or the C-terminal neoepitopes generated in the IGD by the action of MMPs (αG1 MMP) or aggrecanases (αG1 Agg). Control sections in which the primary antibody was replaced by non-immune IgG were also prepared. Representative sections close to the lesion derived from patient 30 are shown. The scale bar indicates 200 μm