Fig. 2

LPS induces proliferation of fibroblast in a dose-dependent manner. Mouse fibroblasts were seeded in 96-well plate. 1 μg, 5 μg, 25 μg/ml LPS were added to various wells; control untreated cells were setup. 48 h later, 20 μl CCK-8 reagent was added into each well for another 2 h. OD450 value was measured to assess cell number. One-way ANOVA with Dunnett's test was used to estimate the differences between the LPS-treated and control groups. The two LPS-treated groups(dot 3 and dot 4)showed more cells with the control group (dot 1). *P < 0.05