Skip to main content
Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping

Fig. 1

Osteoactivin (OA) expression increases in flexor digitorum muscles with high repetition negligible force (HRNF) task. a OA mRNA expression levels (determined using quantitative PCR, qPCR) in muscles of food restricted control (FRC) rats and rats that had performed a HRNF food retrieval task for 3 or 6 weeks. Values were normalized to GAPDH mRNA levels. b A representative Western blot of muscle homogenates probed with anti-OA. Bands are at the expected molecular weights of OA in muscle (approximately 115, 65 and 47 kDa). β-actin was used as the loading control (at 42 kDa). c, d Densitometric analysis of two OA bands (65 and 115 kDa) from three replicate Western blots normalized to β-actin levels. e Quantification of OA-positive immunostaining as a percent of total tissue area. f, g Examples of OA immunostaining in longitudinal sections of FRC and HRNF rat muscles showing OA in the myofiber sarcolemma (arrowheads) and macrophages (mac) only in HRNF muscles. Ct = connective tissue, M = muscle. *p < 0.05, **p < 0.01, compared to FRC rats. Scale bars in F and G = 50 μm

Back to article page