Identification and characterization of human nucleus pulposus cell specific serotypes of adeno-associated virus for gene therapeutic approaches of intervertebral disc disorders

Table 4 Levels of inflammatory, catabolic and matrix proteins in scAAV6 treated NP cells of DDG III

Target protein	NP cell sample	Mean [pg/ml]	SD	Mean fold
IL-1β	control	110	2.247	1.009
IL-1β	scAAV6	109	1.702	1.009
TNF-α	control	93	1.648	1.021
TNF-α	scAAV6	91	2.314	1.021
ADAMTS-4	control	1807	79.49	1.029
ADAMTS-4	scAAV6	1756	105.6	1.029
ADAMTS-5	control	4156	177.9	1.028
ADAMTS-5	scAAV6	4042	203.5	1.028
Aggrecan	control	29100	1112	1.010
Aggrecan	scAAV6	28804	1279	1.010
Collagen II	control	10311	542.7	1.013
Collagen II	scAAV6	10171	548.9	1.013

1 x 10⁵ NP cells were monolayer transduced with scAAV6 [5000 vg/c] and 3D cultured for four weeks in collagen I scaffold. Expression levels [pg/ml] of the inflammatory cytokines (IL-1β & TNF-α), catabolic factors (ADAMTS-4 & ADAMTS-5) and matrix proteins (Aggrecan & Collagen II) were determined using ELISA-assays applied on 100 μg of total protein extracts of each sample. Data represent the mean and standard deviation (SD) of three independent experiments, each performed in duplicate (p < 0.05). The mean fold represents the ratio of the untreated (control) expression level to scAAV6 treated expression level

ISSN: 1471-2474