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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Impact of storage conditions on electromechanical, histological and histochemical properties of osteochondral allografts

Fig. 4

GAG distribution in osteochondral samples, as analyzed by Safranin O-Fast Green staining, and histological–histochemical grading according to Mankin. a Histological images of fresh cartilage and after 14 and 28 days of storage in different conditions. Scale bar = 1 mm. b Quantitative analysis of GAG distribution by red color intensity (RCI) using an adapted image processing protocol. All samples revealed significantly reduced RCI after 28 days of storage. *p < 0.002 (fresh versus all other groups after 28 days); **p < 0.02; ***p ≤ 0.001 (c) Histological-histochemical grading scores. Fresh cartilage was assigned with the score of 0, while higher score indicates cartilage deterioration. ## p < 0.02; # p = 0.005

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