|
Experimental time
|
O’driscoll score parameters
|
CTR
|
IL1β
|
PEMF
|
IL1β + PEMF
|
|---|
|
24 h
|
Safranin-O staining
|
2 (2–3)
|
1 (1–2)**
|
3 (2–3)
|
2 (2–2)§
|
|
Surface regularity
|
2 (2–3)
|
1 (1–2)**
|
3 (3–3)
|
2 (2–2)
|
|
Chondrocyte clustering
|
2 (1–2)
|
0.5 (0–1)**
|
2 (2–2)
|
1 (1–2)
|
|
Cellularity
|
2 (2–3)
|
1 (1–1)**
|
3 (2–3)
|
2 (1–2)§§
|
|
7 days
|
Safranin-O staining
|
1 (1–2)
|
0 (0–1)
|
3 (2–3)°°
|
1 (1–2)§
|
|
Surface regularity
|
2 (1–3)
|
1 (0–1)**
|
3 (2–3)
|
1 (1–2)
|
|
Chondrocyte clustering
|
1 (1–1)
|
0 (0–1)**,a
|
2 (1–2)°°
|
1 (1–2)§
|
|
Cellularity
|
2 (1–2)
|
1 (0–1)**
|
2 (2–3)
|
1 (1–2)
|
|
21 days
|
Safranin-O staining
|
1 (1–2)
|
0 (0–1)
|
2 (2–2)°
|
1 (1–1)§§
|
|
Surface regularity
|
2 (1–2)
|
1 (1–1)**
|
2 (2–2)
|
2 (1–2)§§, b
|
|
Chondrocyte clustering
|
1 (1–1)
|
0 (0–0)*
|
1 (1–1)b
|
1 (1–2)§
|
|
Cellularity
|
1 (1–1)
|
0 (0–1)**,a
|
1.5 (1–2)
|
1 (1–1)§§
|
- Control (CTR), exposed to PEMF (PEMF), treated with IL1β (IL1β) or treated with IL1β and stimulated with PEMF (IL1β + PEMF) explants. 24 h, 7 and 21 days (n = 6)
- Mann-Whitney U test: - Within each experimental time: IL1β versus CTR (*, p < 0.05; **, p < 0.005); PEMF versus CTR (°, p < 0.05; °°, p < 0.005); IL1β + PEMF versus IL1β (§, p < 0.05; §§, p < 0.005); Within each group: 7 days versus 24 h (a, p < 0.05); 21 days versus 7 days (a, p < 0.05; b, p < 0.005)
