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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Hyaluronic acid increases tendon derived cell viability and collagen type I expression in vitro: Comparative study of four different Hyaluronic acid preparations by molecular weight

Fig. 3

Collagen type I, expression of tendon derived cells in vitro culture isolated from 9 healthy patients, and staining as described in material and methods, section Immunofluorescence Staining. Representative images from 4 independent experiments. a. Expression of collagen type I after 7 and 14 d of culture in green fluorescence and in regular light image showing the tendon derived cells. b. Quantization of the immunohistochemistry in a. Synolis-VA® induced the expression of collagen type I significantly after 14 d of in vitro culture. The mean fluorescent intensity/pixel was measured and expressed to the corresponding tendon derived cells. Collagen type I Intensity (Total Area was quantified by anti-collagen type I) it was measured by Nikon software. Data are expressed as mean ± SD for 4 independent experiments for samples run in triplicate. Scale bar (a.): 50 μm

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