Skip to main content
Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: Hyaluronic acid increases tendon derived cell viability and collagen type I expression in vitro: Comparative study of four different Hyaluronic acid preparations by molecular weight

Fig. 2

The apoptosis rate in tendon derived cells. a. The apoptosis was induced with H2O2 (apoptotic control cells [ACtrCs]) and treated with Hyalgan®, Artrosulfur HA®, Hyalubrix® and Synolis-VA® (1000 ug/ml). Apoptosis rate of cells in each sample were detected in a population of 10,000 cells analyzed by flow cytometry. b. The percentage of apoptotic cells in Hyalgan®, Artrosulfur HA®, Hyalubrix® and Synolis-VA® decreased compared to the proliferate control cells (PCtrCs) (without HA). Flow charts: (A1), upper left quadrant annexin V-negative and sytox green-positive cells indicate necrotic cells; (A2), upper right quadrant, annexin V and sytox green -positive cells represent late apoptotic cells. (A3), lower left quadrant annexin V-negative and sytox green-negative cells indicate live cells; (A4), lower right quadrant, annexin V positive and sytox green-negative cells represent early apoptotic cells. Both early and late apoptotic cells were calculated as the incidence of apoptotic cell. The numbers indicate percentage of total gated cells (mean ± S.D.; n = 3). c. % of cells population of live cells, early apoptotic cells, late apoptotic cells and necrotic cells. Graph showing the average % of results obtained in 3 independent experiment. No significant differences were detected (mean ± S.D.; n = 3)

Back to article page
\