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Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Hyaluronic acid increases tendon derived cell viability and collagen type I expression in vitro: Comparative study of four different Hyaluronic acid preparations by molecular weight

Fig. 1

Effects of Hyalgan®, Artrosulfur HA®, Hyalubrix® and Synolis-VA® on morphologic change cell viability and cell metabolic activity. Tendon derived cells were treated with different concentrations of Hyalgan, Artrosulfur HA®, Hyalubrix® and Synolis-VA® for different time periods. a. after treatment for 14 d the morphology of the cells treated with all the HAPs was photographed (1000ug/ml). Magnification, ×100. b. Absolute number of live cells was calculated using trypan blue exclusion. c. Cell metabolic activity was evaluated with the Alamar Blue method. All tests and determinations were repeated in triplicate. The metabolic activity rate was calculated by subtracting the background OD value (complete culture medium without cells) from the OD value from each test well (see materials and methods section). Hyalgan®, Artrosulfur HA®, Hyalubrix®, Synolis-VA® increased cell activity in a concentration-dependent pattern and with regard to time. No statistically significant difference were observed from control according to Student's t test

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