Fig. 7

Periostin-induced upregulation of catabolic enzymes and inflammatory cytokines via the NF-κB signaling. (a) Human primary chondrocytes were stimulated by 20 μg/mL periostin, and the nuclear translocation of p65 was determined by immunocytochemistry at 3 h. Gray and green represent p65. Red represent nucleus. Bar = 10 μm. (b) Human primary chondrocytes were exposed to 20 μg/mL periostin and/or different concentrations of BAY11-7082 for 24 h. The mRNA levels of IL-6, IL-8, MMP-1, MMP-3, MMP-13, and NOS2 were measured by quantitative RT-PCR. The relative expression compared to vehicle control is shown. Representative data from one of three donors are shown. *, P < 0.05