Fig. 2
From: Expression and pathological effects of periostin in human osteoarthritis cartilage
Periostin localization in human OA cartilage. Toluidine blue staining (a, c, e) and periostin immunostaining (b, d, f, b, d, f) of human osteoarthritis (OA) cartilage. Right panels (b, d, f) are high magnification images of the middle panels (b, d, f), respectively. The inset in the right panels shows the isotype control. The different OA grade samples are shown; near the intact cartilage (a, b, b) (Mankin score, 1; Positive cell rate, 0; Intensity, 0), moderate OA cartilage (c, d, d) (Mankin score, 8; Positive cell rate, 4; Intensity 1), and severe OA cartilage (e, f, f) (Mankin score, 11; Positive cell rate, 6; Intensity 2). In denatured cartilage, periostin was localized in chondrocytes and their periphery matrix particularly near the erosive surface. Bars = 100 μm. Positive correlation between IHC score (g) and Mankin score was observed (h). (r = 0.649, P < 0.001)