Fig. 7
Effect of α-MSH on NO secretion and iNOS gene expression in IL-1β or TNF-α-stimulated chondrocytes. Human primary chondrocytes were incubated for 4 h at 37 °C with 10−6M α-MSH and further cultured for 40 h in the presence or absence of 10 ng/mL IL-1β or TNF-α. NO concentration in the supernatans (a) and iNOS gene expression (b) were then measured. Data from 4 separate experiments (± S.E.M.) are shown. *p < 0.0001 vs Medium; §p < 0.005 vs IL-1β; °p < 0.005 vs TNF-α