Fig. 4

Caspase-3, Bax and Bcl-2 expression. a: Caspase-3 activity in the femoral head bone tissue. *p < 0.05 vs. group A; **p < 0.01 vs. group A; and ^##p < 0.01 vs. group B. b: Western blotting of Bax and Bcl-2. c: OD ratios for BAX. *p < 0.05 vs. group A; **p < 0.01 vs. group A; and ^#p < 0.05 vs. group B. d: OD ratios for Bcl-2. *p < 0.05 vs. group A; **p < 0.01 vs. group A; and ^##p < 0.01 vs. group B. e: Immunohistochemical staining of Bax. Positive staining for Bax was observed in groups B and C (magnification 200×). f: Immunohistochemical staining of Bcl-2. Positive staining for Bcl-2 in groups B and C was significantly weaker than that in group A (magnification 200×)