Fig. 1
From: Type II collagen and glycosaminoglycan expression induction in primary human chondrocyte by TGF-β1
TGF-β1 produced from hChonJb#7 functions as rhTGF-β1. a TGF-β1gene was modified to bear WT or mutants related to hChonJb#7. Its expression was tested by western blot using culture medium of Cos-1 cells that were transfected with individual constructs. 1: rhTGF-β1, 2: pMSCV TGF-β1 (WT), 3: pMSCV TGF-β1 (S223/225 mutant), 4: pMSCV TGF-β1 (A84/W255 mutant), 5: pMSCV TGF-β1 (S223/225 & A84/W255 mutant, designated #7). Upper panel is showing matured dimer of TGF-β1in a non-reducing gel that was shifted into monomer form in a reducing gel (lower panel). b TGF-β1gene transfected Cos-1 cell culture medium or hChonJb#7 cell culture medium was used as TGF-β1source to inhibit proliferation of Hep G2 carcinoma cells in 5 ng/ml concentration. Control; no addition of any TGF-β1, WT; wild type TGF-β1, S223/225; Serine 223 and Serin225 changed mutant TGF-β1, A84/W255; Threonine 84 and Arginine 255 changed mutant TGF-β1, #7; combined mutant of S223/225 and A84/W255, hChonJb#7; TGF-β1 contained in hChonJb#7 culture medium. The absorbance of the Y-axis increases in proportion to the number of live cells. The error bars represent the standard deviation. c The serially diluted supernatant containing TGF-β1 in concentration of 10 ng/ml to 0.156 ng/ml was applied into Hep G2 cell culture to determine IC50 of each TGF-β1 variant