Skip to main content
Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures

Fig. 3

Time-series analysis to identify clusters of expression associated with immobilization. Posterior knee capsule samples from sham-operated and immobilized rats joints were harvested at time points of 1, 2, 4, 8, and 16 weeks and were subjected to genome-wide microarray analysis. Expression values from the sham and immobilized samples were analyzed separately to quantify fold changes and to cluster genes with similar profiles of expression. The two lists of genes included in clusters generated from temporal expression analysis were compared to identify similarities. Genes unique to immobilization were analyzed in depth to gain insight on the biological significance by integration of CAGED, GO, KEGG, Reactome, and literature biocuration results. Immunohistochemistry was used to validate the microarray results for selected genes

Back to article page