Representative images of the measurements made on undecalcified sections. (A) cartilage and subcondral bone cortical thickness (nCg.Th; cCg.Th; Cg.Th = nCg.Th + cCg.Th) SB.Th). (B). Surface undulation (FI). (C). Trabecular subchondral bone measurements in a ROI. Tb.A: % of trabecular bone in the ROI; Tb.Sp measured on the diagonal of the ROI (Tb.Sp = (1/Tb.N)-Tb.Th).