Levels of serum biomarkers. COMP, CTX-II, MMP-3, PINP and TRAP-5b were measured by ELISA. Serum levels of OC, OPG and RANKL were determined by luminex. ALP levels in serum were determined as previously described . Results show mean ± SD (n = 12-15); +P < 0.05, ++P < 0.01 with respect to Sham; *P < 0.05, **P < 0.01compared to Control (ANOVA followed by Bonferroni’s test).