Table 1 List of genes and oligonucleotide sequences of the primers used for real-time RT-PCR
Gene | Primers: 5′-3′ | Access number |
|---|---|---|
GAPDH | Foward: CAACTCCCTCAAGATTGTCAGCAA | NM 002046 |
Reverse: GGCATGGACTGTGGTCATGA | ||
Type 1 collagen | Foward: ACGTCCTGGTGAAGTTGGTC | NM 000088 |
Reverse: CAGGGAAGCCTCTTTCTCCT | ||
Osteocalcin | Foward: CATCTATGGCACCACCGTTT | NM 013414.1 |
Reverse: AGAGAGAGGGAACAGGGAGG | ||
Bone sialoprotein | Foward: TGTCCTTCTGAACGGGTTTC | NM 012587.2 |
Reverse: CTTCCCCATACTCAACCGTG | ||
Osteopontin | Foward: ATCTCACCATTCCGATGAATCT | AB001382 |
Reverse: TCAGTCCATAAGCCAAGCTATCA | ||
Alkaline Phosphatase | Foward: CTAGTTCCTGGGAGATGGTA | AC_000073.1 |
Reverse: GTGTTGTACGTCTTGGAGAGA | ||
Runx-2 | Forward: GCGTCAACACCATCATTCTG | NM_004348 |
Reverse: CAGACCAGCAGCACTCCATC |