Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy

Table 1 List of genes and oligonucleotide sequences of the primers used for real-time RT-PCR

Gene	Primers: 5′-3′	Access number
GAPDH	Foward: CAACTCCCTCAAGATTGTCAGCAA	NM 002046
GAPDH	Reverse: GGCATGGACTGTGGTCATGA	NM 002046
Type 1 collagen	Foward: ACGTCCTGGTGAAGTTGGTC	NM 000088
Type 1 collagen	Reverse: CAGGGAAGCCTCTTTCTCCT	NM 000088
Osteocalcin	Foward: CATCTATGGCACCACCGTTT	NM 013414.1
Osteocalcin	Reverse: AGAGAGAGGGAACAGGGAGG	NM 013414.1
Bone sialoprotein	Foward: TGTCCTTCTGAACGGGTTTC	NM 012587.2
Bone sialoprotein	Reverse: CTTCCCCATACTCAACCGTG
Osteopontin	Foward: ATCTCACCATTCCGATGAATCT	AB001382
Osteopontin	Reverse: TCAGTCCATAAGCCAAGCTATCA	AB001382
Alkaline Phosphatase	Foward: CTAGTTCCTGGGAGATGGTA	AC_000073.1
Alkaline Phosphatase	Reverse: GTGTTGTACGTCTTGGAGAGA	AC_000073.1
Runx-2	Forward: GCGTCAACACCATCATTCTG	NM_004348
Runx-2	Reverse: CAGACCAGCAGCACTCCATC	NM_004348

ISSN: 1471-2474