Figure 3
Percentage of mineralization nodules/field (mean ± standard deviation) (a) and Von Kossa staining (b) of calvarial osteoblasts taken from newborn control rats and those exposed to various concentrations of caffeine (25, 50, 100 mg/Kg) during pregnancy and subsequently cultured in osteogenic differentiation medium for 21 days. ***p < 0.001. Bar = 58.5 μm.