Figure 2
Alkaline phosphatase activity (a) and synthesis of collagen (b) (mean ± standard deviation) in calvarial osteoblasts taken from newborn control rats or those exposed to various concentrations of caffeine (25, 50, 100 mg/Kg) during pregnancy and subsequently cultured in osteogenic differentiation medium for 7, 14 or 21 days. *p < 0.05, **p < 0.01, ***p < 0.001.