Small changes in bone structure of female α7 nicotinic acetylcholine receptor knockout mice

Table 1 Primer pairs used for real-time RT-PCR

Primer	Sequence	Length[bp]	Annealing temp.[°C]	GenBank ID(accession)
Col1α1^a for	TGCCTGGCGGACTTCCTGGT	144	62	NM_007742.3
rev	CCGGCACCTGGCTTAGGTGG	144	62	NM_007742.3
Col1α2^b for	ATGCCGGTCGACCTGGGGAA	144	60	NM_007743.2
rev	GTCCAGCTGGGCCGATTGGG	144	60	NM_007743.2
CtsK^c for	GAGGCGGCTATATGACCACT	119	58	NM_007802.3
rev	CTTTGCCGTGGCGTTATACA	119	58	NM_007802.3
Bglap^d for	TTCTGCTCACTCTGCTGACC	111	58	NM_007541.2
rev	TATTGCCCTCCTGCTTGGAC	111	58	NM_007541.2
ALP^e for	TTAAGGGCCAGCTACACCAC	150	58	NM_007431.2
rev	CCTTCACGCCACACAAGTAG	150	58	NM_007431.2
βActin for	TGTTACCAACTGGGACGACA	165	58	NM_007393.3
rev	GGGGTGTTGAAGGTCTCAAA	165	58	NM_007393.3

^aCol1α1: Collagen 1α1, ^bCol1α2: Collagen 1α2, ^cCtsK: Cathepsin K, ^dBglap: Osteocalcin, ^eALP: alkaline phosphatase.

ISSN: 1471-2474