Table 1 Primers using the characteristics of SP cells and differentiated cells

β-actin

280979

AGGTCATCACCATCGGCAAT; ex 3*

GAATGCCGCAGGATTCCAT; ex 4

87 bp

CD34

281051

TGCTATTTCCTGATGAACCGC; ex 7

TCCACGTAATAAGGGTCTTCGC; ex 8

74 bp

Flk-1

407170

TTCCAAGTGGCTAAGGGCAT; ex 22

TTTAACCACGTTCTTTTCCGACA; ex 23

102 bp

c-Kit

280832

CAAGGAAGGTTTCCGAATGC; ex 19

CCCAGCAGGTCTTCATGATGT; ex 20

74 bp

Abcb-1 (Mdr-1)

281585

GCAAAGCAGGCGAGATCC; ex 17

TCAATGCTCCCGTGGTGTT; ex 18

111 bp

Abcg-2 (Bcrp-1)

536203

CCTTGGTTGTCATGGCTTCA; ex 14

AGTCCTGGGCAGAAGTTTTGTC; ex 15

98 bp

Pcgf-4 (Bmi-1)

510666

GGCTGGAACCGCCTAAAAC; ex 1

TCTGCTTGATAAAAGATCGGCTC; ex 1–2

91 bp

COL1A1

282187

ATGGCGAAGCTGGAAAG; ex 9–10

CCACTGAAACCTCTGTGT; ex 11–12

121 bp

COL2A1

282189

TGGTATCGCCGGACCCAAG**

CTCGTCCACCGTCCTTCCC**

82 bp

Aggrecan

280985

CACCTGTAAAAAGGGCACAGTG; ex 15

GCATTGATCTCGTATCGGTCC; ex 16

95 bp

CD-RAP

280857

TGACCGGAAGATGTGTGCC; ex 2

CACGTAGTCCTGAAGGGCCA; ex 3

73 bp

MyoD-1st

281938

CAAACGCAAGACGACTAACG; ex 1

TGTAGTAAGTGCGGTCGTAG; ex 2

392 bp

MyoD-2nd

281938

AACGCCATCCGCTATATCG; ex 1

TGTAGTCCATCATGCCGTCG; ex 2

187 bp

Myf-5

281335

ACATTGAGAGTCTGCAGGAG; ex 1

TTGCTCTGAGTTGGTGATC; ex 3

269 bp

Osteocalcin

281646

AGATGCAAAGCCTGGTGATG; ex 2

ATGTGGTCAGCTAGCTCGTC; ex 4

192 bp