Figure 5From: Isolation and characterization of side population stem cells in articular synovial tissue Evaluation of differentiation properties of SP cells. (A) In vitro chondrogenesis. SP and NSP cells were cultured for 21 days in TGF-β contained chondrocyte differentiation medium. (A-a) Pellets were stained with toluidine blue and alcian blue. (A-b) Expression of chondrogenic genes was also examined. SP and NSP cells were cultured for 21 days, and chondrocyte specific gene expressions were determined by RT-PCR analysis. Representative data were shown with 3 experiments. (B) In vitro osteogenesis. SP and NSP cells were cultured for 21 days in osteocyte differentiation medium. (B-a) ALP activity was determined in both derivatives. (B-b) Calcium deposition was examined by 1% Alizarin red staining, High-magnification image of stained cells derived from SP cells were shown in right panel. Scale bar is 200 μm. (B-c) Expression of osteocyte specific gene Osteocalcin was determined by RT-PCR. (C) In vitro myogenesis. SP and NSP cells were cultured for 7 days in myocyte differentiation medium. Desmin expressions were determined by immunocytochemical staining (C-a; scale bar = 100 μm), and high-magnification image of SP derived myocyte (C-b; scale bar = 50 μm). (C-c) Myocyte specific gene expressions were determined by RT-PCR.Back to article page