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Figure 4 | BMC Musculoskeletal Disorders

Figure 4

From: Mechanism of HIV protein induced modulation of mesenchymal stem cell osteogenic differentiation

Figure 4

Effect of hiv proteins on non differentiating MSCs. MSCs were cultured in 96-well plates in the presence of 100 ng/ml HIV p55-gag and REV for 7 days. Untreated cells were included as controls. Cells were harvested and the stained for alkaline phosphatase, calcium deposition, and lipid accumulation (Fig. 4). Following staining dyes were extracted, the degree of staining quantified spectrophotometrically and normalised to cell number. Data expressed percentage of untreated control ± SEM. All data is the mean of several independent experiments. (Significance determined at; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005)

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