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Figure 1 | BMC Musculoskeletal Disorders

Figure 1

From: Mechanism of HIV protein induced modulation of mesenchymal stem cell osteogenic differentiation

Figure 1

Validation of whole cell elisa protocol for ICAM, and CTGF. Fig. S1 A, B; standard curves for cell # vs. OD for ponceau red (protein) and methylene green (DNA) respectively. Fig. S1 C, D; time and dose response curves for ICAM detected by whole cell elisa vs. TNFα. Fig. S1E; CTGF levels as measured by whole cell ELISA vs. DEX and TGF-β concentration. Fig. S1A, B; data is presented as zeroed OD ± SEM. Fig. S1. C, D, E, F; data is presented as fold change relative to untreated control ± SEM. All data is the mean of several independent experiments. (Significance determined at; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005.)

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