Figure 3From: Bovine explant model of degeneration of the intervertebral disc(a) H&E stained sections at 3 weeks post-incubation with 20 mg trypsin, 20 mg papain or no enzyme (control). (b) Toluidine blue stained sections demonstrating severe loss of metachromasia from all of the disc in enzyme treated discs compared to non-enzymatically digested, where loss is diminished and only in the outer annulus.Back to article page