Table 1 Primer nucleotide sequences used in RT-qPCR assays for genes described in the study
From: Differential gene expression associated with postnatal equine articular cartilage maturation
Gene Name | Gene Symbol | Forward Primer | Reverse Primer |
|---|---|---|---|
Beta-2-microglobulin | B2M | 5-CGGGCTACTCTCCCTGACT-3 | 5-GTGACGTGAGTAAACCTGAACCTT-3 |
Beta-glucuronidase | GUSB | 5-GCTCATCTGGAACTTTGCTGATTTT-3 | 5-CTGACGAGTGAAGATCCCCTTTT-3 |
Phosphoglycerate kinase-1 | PGK1 | 5-GTGGCCTCTGGCATACCT-3 | 5-CTGCTCTCAGTGCCACAGT-3 |
Ribosomal protein, large, P0 | RPLP0 | 5-CTGATTACACCTTCCCACTTGCT-3 | 5-AGCCACAAATGCAGATGGATCA-3 |
Procollagen, type II, alpha 1 | COL2A1 | 5-CTGGCTTCAAAGGCGAACAAG-3 | 5-GCACCTCTTTTGCCTTCTTCAC-3 |
Cartilage oligomeric matrix protein | COMP | 5-CGAGCCCGGCATCCA-3 | 5-CCCAGGGCCTGTGGAG-3 |
Proline-4-hydroxylase, alpha 1 | P4HA1 | 5-CCTGTCCAGTGCTAGTAGGAAAC-3 | 5-CCTGTCCAGTGCTAGTAGGAAAC-3 |
Transforming growth factor beta-1 | TGFβ1 | 5-CCCTGCCCCTACATTTGGA-3 | 5-TGTACAGGGCCAGGACCTT-3 |
Transforming growth factor beta receptor 3 | TGFβR3 | 5-ACATCATCGAGTTAATCACCCCAAA-3 | 5-GATCCTTTCGAGAAGGTCTTATGTCA-3 |
Tenascin-C | TNC | 5-TCAGCCATCACTACCAAGTTCAC-3 | 5-GAACCTCAGTAGCAGTCAAATCTCT-3 |