Figure 2From: Activation and localization of matrix metalloproteinase-2 and -9 in the skeletal muscle of the muscular dystrophy dog (CXMDJ) Immunohistochemical analysis of CD11b or MHCd expression, and film in situ zymography in the presence and absence of an MMP inhibitor (FIZ-PT) in the skeletal muscle of CXMD J dogs. A-D: serial sections of CXMDJ muscle characterized by extensive necrosis and an invading inflammatory infiltrate; E-H: serial sections of groups of regenerating fibers in CXMDJ muscle. A and B: H&E staining; B and F: immunohistochemical analysis of CD11b; C and G: FIZ-GN; D and H: FIZ in the presence of an MMP inhibitor (1,10-phenanthrolone) (FIZ-PT). Gelatinolytic activity co-localized with CD11b and MHCd, immunoreactivity and was attenuated by adding 1,10-phenanthrolone to the FIZ assay system. Bars, 50 μm.Back to article page