Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell differentiation study

Table 1 Primer and probe nucleotide sequences of the tested genes

Gene	Accession no.	Primer	Probe
GAPDH	NM_002046.2	F: ATGGGGAAGGTGAAGGTCG R: TAAAAGCAGCCCTGGTGACC	CGCCCAATACGACCAAATCCGTTGAC
RUNX2	NM_001024630.1 NM_00101505.1 NM_0043468.3	F: GCCTTCAAGGTGGTAGCCC R: CGTTACCCGCCATGACAGTA	CCACAGTCCCATCTGGTACCTCTCCG
BGLAP	NM_199173.2	F: GAAGCCCAGCGGTGCA R: CACTACCTCGCTGCCCTCC	TGGACACAAAGGCTGCACCTTTGCT
PPARG*	NM_138712.2 NM_015869.3 NM_005037.4 NM_138711.2	F: AGGGCGATCTTGACAGGAAA R: TCTCCCATCATTAAGGAATTCATG
SOX9	NM_000346.2	F: CAACGCCGAGCTCAGCA R: TCCACGAAGGGCCGC	TGGGCAAGCTCTGGAGACTTCTGAACG
AGC1	NM_001135.1 NM_013227.1	F:TCGAGGACAGCGAGGCC R:TCGAGGGTGTAGCGTGTAGAGA	ATGGAACACGATGCCTTTCACCACGA
COL2A1	NM_033150	F: GGCAATAGCAGGTTCACGTACA R: CGATAACAGTCTTGCCCCACTT	CCGGTATGTTTCGTGCAGCCATCCT
COL10A1	NM_000493.2	F: CAAGGCACCATCTCCAGGAA R: AAAGGGTATTTGTGGCAGCATATT	TCCAGCACGCAGAATCCATCTGA

F: forward; R: reverse; * SYBR^®Green assay. Commercially available, so-called assays-on-demand (Applied Biosystems, Foster City, CA, U.S.A.) were used to detect osterix (SP7; Hs_00541729_m1) and fatty acid binding protein 4 (FABP4; Hs_00609791)-specific mRNA.

ISSN: 1471-2474