Table 1 Primer sequences and sizes of amplicons generated by Real-time RT-PCR
mRNA | Forward primer (5'-3') | Reverse primer (5'-3') | Annealing [°C] | Amplicon [bp] | Accession number | Citation |
|---|---|---|---|---|---|---|
BMP-2 | ATGGATTCGTGGTGGAAGTG | GTGGAGTTCAGATGATCAGC | 58 | 349 | NM_001200.2 | [41] |
BMP-4 | AGCATGTCAGGATTAGCCGA | TGGAGATGGCACTCAGTTCA | 58 | 399 | NM_130851.1 | [41] |
BMP-6 | CAGCCTGCAGGAAGCATGAG | CAAAGTAAAGAACCGAGATG | 53 | 246 | NM_001718.2 | [42] |
β-actin | GGGTCAGAAGGATTCCTATG | GGTCTCAAACATGATCTGGG | 58 | 237 | NM_001101.2 | [43] |