Figure 4
From: Dexamethasone stimulates expression of C-type Natriuretic Peptide in chondrocytes
DEX increases Prkg2 expression in chondrocytes. Primary chondrocytes were incubated with DMSO or 10-7 M DEX for 12 to 72 hours, and Prgk1 (encoding cGMP-dependent protein kinase I, A) and Prkg2 (encoding cGMP-dependent protein kinase II, B) mRNA levels were determined by real-time PCR relative to Gapdh. While Prkg1 expression remained relatively unchanged over time and in response to DEX treatment, Prkg2 levels were significantly increased during the time course and in response to DEX. Data represent mean ± SEM from three or four independent trials (*P < 0.05).