Figure 5From: Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK Effect of hydrostatic pressure on the percentage of TRAP positive cells. MP (5 × 105/ml) from healthy volunteers were cultured at 5 × 105 cells/ml on Thermanox coverslips in supplemented medium for 7 days before pressurisation (34.5 × 10-3MPa) (5.0 psi) for four cycles of pressure. MP were fixed and stained for TRAP. For each sample, the number of positive and negative cells in 5 random microscope fields was counted, for each of three replicates. Results are the mean and SD of 5 separate experiments.Back to article page