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Figure 4 | BMC Musculoskeletal Disorders

Figure 4

From: Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK

Figure 4

Effect of hydrostatic pressure on the number of TRAP positive and multinucleated cells. MP (5 × 105/ml) from healthy volunteers were cultured at 5 × 105 cells/ml on Thermanox coverslips in supplemented medium for 7 days before pressurisation (34.5 × 10-3MPa) (5.0 psi) for four cycles of pressure. MP were fixed and stained for TRAP; a) with pressure; b) without pressure. More multinucleated cells (arrows) were seen with pressure c) than without d).

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