Figure 4From: Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK Effect of hydrostatic pressure on the number of TRAP positive and multinucleated cells. MP (5 × 105/ml) from healthy volunteers were cultured at 5 × 105 cells/ml on Thermanox coverslips in supplemented medium for 7 days before pressurisation (34.5 × 10-3MPa) (5.0 psi) for four cycles of pressure. MP were fixed and stained for TRAP; a) with pressure; b) without pressure. More multinucleated cells (arrows) were seen with pressure c) than without d).Back to article page