Transforming growth factor beta-1 (TGFB1) and peak bone mass: association between intragenic polymorphisms and quantitative ultrasound of the heel

Table 1 Primer sequences used for the amplification of polymorphic TGFB1 sites.

	Downstream Primer	Upstream Primer	Amplicon Size (bp)	Enzyme^a	Cleavage^b
-800				Eco1 81I	Wild-type
AGAACAGTTGGCACGGGCTT	AACGGAAGAGAGTCAGGCT	583
-509				Tai I	Wild-type
Codon10				MspA 1I	Wild-type
CTACCTTTTGCCGGGAGACC	CACCAGCTCCATGTCGATAG	226
Codon25	CTTTCGCCTTAGCGCCCACT	TAGTTGGTGTCCAGGGCTCG	343	Sau 961	Wild-type
Codon 263				Fok 1	Wild-type
713-8delC				Bse L1	Variant

^aRestriction endonucleases (Enzyme) are shown with digestions conducted separately. ^bAllele recognition (and cleavage) for either the major (Wild-type) or minor (Variant) allele.

ISSN: 1471-2474