Figure 8

Morphology of DD cells under isometric tension in FPCLs. Depicted are attached (A) FPCL cultures of one representative patient-matched disease and control primary cell line (DUP36). FPCLs were harvested at the indicated attached time points (A0 – A48 hours). Lattices were fixed and stained for F-actin and DNA, using phalloidin-Alexa 488 (green) and DAPI (blue), respectively (Molecular Probes Eugene ON, USA). Digital images were acquired on a Nikon eclipse TE-200 inverted fluorescent microscope (10x objective) using a Photometrics series 300 cooled CCD camera. The scale bar denotes 100 μ.