Figure 7
Western analysis of β-catenin and Fn in attached FPCLs. Attached FPCLs (3 lattices per cell line) were harvested at the indicated time points, homogenized, and protein extract prepared for western analysis. Membranes were sequentially probed with β-catenin (1:750; clone 14, Transduction Laboratories), Fn (clone IST-4, 1:500, Sigma), and Hsp47 (1:500, StressGen) antibodies. Antibody-specific bands for β-catenin and fibronectin and Hsp47 were quantified using NIH Imaging software, normalized to Hsp47 levels (ratios), and plotted (bar graphs, lower panel) as the mean ratio intensity ± SEM per time point. Two patient-matched disease and normal/control primary cultures were examined.