Figure 6

Western analysis of β-catenin and Fn in contracting FPCLs. The upper panel shows the western analysis results of contracting 'stressed' FPCLs of three patient-matched disease (D) and normal/control (C) primary cell lines (n = 3). FPCLs (3 lattices per cell line) were harvested at the indicated time points following mechanical release and homogenized for protein extraction. The resulting western blots were sequentially probed with β-catenin (1:750; clone 14, Transduction Laboratories), and Fn (clone IST-4, 1:500, Sigma) and Hsp47 (1:500, StressGen) antibodies. Antibody-specific bands for β-catenin and fibronectin and Hsp47 were quantified using NIH Imaging software, normalized to Hsp47 levels (ratio), and plotted (bar graphs, lower panel) as the mean ratio intensity ± SEM per time point.