Figure 5

ICC of FPCL cultures. Depicted are representative images from disease (d) and normal/control (c) matched primary culture (patient DUP48). The cells were grown as attached FPCLs for 2 days (10⁵ cells/ml of matrix). Cells were then fixed and stained with phalloidin-Alexa 488 (green) and DAPI (blue) to label both F-actin and the nucleus, respectively. Digital images were acquired on a Nikon eclipse TE-200 inverted fluorescent microscope (10x objective) using a Photometrics series 300 cooled CCD camera, and deconvolved using softWoRx (v 2.5) software (Applied Precision Inc., Issaquah WA, USA). Arrows identify prominent F-actin networks (stress fibres) within the diseased cells. The scale bar denotes 100 μ.