Figure 2

Cultures stained with TRAP stain and DNA-binding fluorochrome Hoechst 33258. The appearance of the cultures is dramatically changed when gap-junctional communication is blocked by either of the inhibitors. (A, B) Control cultures stained with TRAP-stain and Hoechst 33258 with numerous healthy-looking TRAP-positive cells with intact nuclei. (C, D) Heptanol-treated cultures as well as (E, F) Gap 27 – treated cultures have fewer TRAP-positive cells. Image width in figures 2A, C and 2E is 400 μm and in figures 2B, 2D and 2F 200 μm.