Effect of HBO on the expression of ATP6V0 and Wnt3a. Protein levels of ATP6V0 were up-regulated after HBO treatment in the cell lysates (1.71 ± 0.12-fold, p < 0.01, A) and the effect of HBO was reduced by ATP6V0 siRNA treatment (1.71 ± 0.12-fold vs. 0.72 ± 0.07-fold, p < 0.01, A). The amounts of Wnt3a in the cell lysates (1.89 ± 0.07-fold, p < 0.01, B) and conditioned culture medium (I vs. IH, *p < 0.05, C) were both up-regulated after HBO treatment. The effect of HBO on secreted Wnt3a was reduced (IH vs. IH + siRNA, **p < 0.01, C) but the intracellular Wnt3a levels were not affected (IH/I vs. IH + siRNA/I, p > 0.05, n = 3; B) by ATP6V0 siRNA treatment. Each bar represents the value of the mean ± SD and analyzed by Student t-test (I, induction; IH, induction + HBO).