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Figure 5 | BMC Musculoskeletal Disorders

Figure 5

From: Effects of hyperbaric oxygen on the osteogenic differentiation of mesenchymal stem cells

Figure 5

Long-term effects of HBO on MSCs. (A) HBO significantly increased alkaline phosphatase activity after 7 d (*p < 0.05, n = 4, Student’s t-test), 14 d (**p < 0.01, n = 4, Student’s t-test), and 21 d (**p < 0.01, n = 4, Student’s t-test) of culturing. (B) HBO significantly increased calcium levels after 14 d (*p < 0.05, n = 4, Student’s t-test) and 21 d (**p < 0.01, n = 4, Student’s t-test) of culturing. (C) Greater positive von Kossa staining through the matrix at the surface layer of the HBO group was observed compared to the control group (1.473 ± 0.23-fold, p < 0.05, n = 3, Student’s t-test).

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