Figure 4

Effect of HBO on β-catenin translocation in MSCs. The protein levels of β-catenin in the nuclear fractions were up-regulated after HBO treatment (2.91 ± 0.75-fold, p < 0.05, n = 3, Student’s t-test, A). No significant difference in the cytoplasmic fractions was shown after HBO treatment (1.04 ± 0.22-fold, p > 0.05, n = 3, Student’s t-test, A). The increased protein levels of β-catenin (A) and Runx2 (B) by HBO treatment were all down-regulated by β-catenin siRNA treatment.