Figure 2

Effect of HBO on mRNA expression by MSCs. The mRNA ratios of Wnt3a (A, II / I, **p < 0.01, n = 3, Student’s t-test), β-catenin (A, II / I, *p < 0.05, n = 3, Student’s t-test), and Runx2 (A, II / I, *p < 0.05, n = 3, Student’s t-test) were up-regulated, while GSK-3β (A, II / I, *p < 0.05, n = 3, Student’s t-test) was down-regulated after HBO treatment. The mRNA levels of β-catenin (B, II / I, *p < 0.05, n = 4, Student’s t-test) and Runx2 (C, II / I, *p < 0.05, n = 4, Student’s t-test) were up-regulated after HBO treatment. The silencing effect on β-catenin (B, II vs. III, **p < 0.01, n = 4, Student’s t-test) and downregulating effect for Runx2 (C, II vs. III, *p < 0.05, n = 4, Student’s t-test) by β-catenin siRNA were detected after the treatments. No significant difference was shown between II and IV (***p > 0.05, n = 4, Student’s t-test, B, C). (I, induction; II, induction + HBO; III, Induction + HBO + siRNA; IV, Induction + HBO + scrambled siRNA).