Cx43 Knockdown in human synovial fibroblast-like cells reduced the expression of OA-associated catabolic and inflammatory genes. By quantitative real time RT-PCR, transient transfection of SW982 human synovial fibroblast-like cells with GJA1 targeting siRNAs (GJA1) reduced the gene expression of some OA-associated (A) catabolic genes and (B) inflammatory genes relative to a scrambled siRNA control (SCR) N = 3. Data are shown as means ± standard deviations. Asterisks indicate p-value < 0.05 relative to the empty vector control. (C) Immunoblotting of transfected SW982 cells confirmed a decrease in Cx43 expression in GJA1 siRNA transfected cells relative to cells transfected with a scrambled control siRNA (SCR). GAPDH was used as a load control.