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Table 2 Summary of mass spectrometric analysis of the most interesting proteins related to the IVD extracellular matrix homeostasis identified in serum free medium (SFM) on day 0, conditioned medium (NCCM) of pure NC collected after 7 days and co-culture (CoC) medium of 1:1 NC: NPC co-culture (CoC) after 7 days CoC

From: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

ID Full name SFM NCCM CoC 1:1 cnts
ECM      
CO1A1_BOVIN collagen type 1 A1 chain 20.0 46.4 33.6 3
CO1A2_BOVIN collagen type 1 A2 chain 23.4 40.1 36.5 3
CO5A1_HUMAN Collagen alpha-1(V) chain 24.0 40.0 36.0 3
CO6_BOVIN Collagen alpha-1(VI) chain 57.1 42.9 0.0 2
COMP_HUMAN Cartilage oligomeric matrix protein (COMP) 26.8 36.6 36.6 3
Cytokines      
CTGF_PIG Connective tissue growth factor 0.0 40.0 60.0 2
CXL16_PIG C-X-C motif chemokine 16 0.0 0.0 100.0 1
GALA_BOVIN Galanin message-associated peptide (GMAP) 0.0 0.0 100.0 1
IBP2_PIG Insulin-like growth factor-binding protein 2 (IBP-2) 0.0 61.8 38.2 2
IBP5_HUMAN Insulin-like growth factor-binding protein 5 (IBP-5) 6.8 0.0 93.2 2
IL6_BOVIN Interleukin-6 (IL-6) [CHAIN 0] 0.0 0.0 100.0 1
Metalloproteinases     
MMP1_HORSE Interstitial collagenase (MMP-1) 0.0 100.0 0.0 1
MMP1_PIG 18 kDa interstitial collagenase 0.0 86.5 13.5 2
MMP2_BOVIN PEX 0.0 0.0 100.0 1
Inhibitors of Metalloproteinases     
TIMP1_BOVIN Metalloproteinase inhibitor 1 (EG-1, TIMP-1) 0.0 0.0 100.0 1
TIMP1_PIG Metalloproteinase inhibitor 1 (TIMP-1) 0.0 47.4 52.6 2
Proteases      
HTRA1_HUMAN Serine protease HTRA1 0.0 0.0 100.0 1
Other      
ALBU_PIG Serum albumin 0.0 59.6 40.4 2
PRDX1_HUMAN Peroxiredoxin-1 (NKEF-A) (PAG) 0.0 100.0 0.0 1
PRG4_HUMAN Proteoglycan 4 C-terminal part [ISOFORM E] 0.0 0.0 100.0 1
SAA1_MUSVI Serum amyloid A-1 protein 0.0 100.0 0.0 1
SAA2_MUSVI Amyloid protein A [CHAIN 0] 0.0 78.0 22.0 2
CLC11_HUMAN C-type lectin domain family 11 member A 0.0 0.0 100.0 1
  1. SFM = serum free medium control, CoC 1:1 = NPC: NC Co-Culture, NCCM = Conditioned Medium.
  2. The numbers indicate peptide spectrum matches, expressed as percentage from the total over all three media. Cells were kept under normoxia.
  3. Number in bold are percentages > 33%.
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