Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

Table 2 Summary of mass spectrometric analysis of the most interesting proteins related to the IVD extracellular matrix homeostasis identified in serum free medium (SFM) on day 0, conditioned medium (NCCM) of pure NC collected after 7 days and co-culture (CoC) medium of 1:1 NC: NPC co-culture (CoC) after 7 days CoC

ID	Full name	SFM	NCCM	CoC 1:1	cnts
*ECM*
CO1A1_BOVIN	collagen type 1 A1 chain	20.0	46.4	33.6	3
CO1A2_BOVIN	collagen type 1 A2 chain	23.4	40.1	36.5	3
CO5A1_HUMAN	Collagen alpha-1(V) chain	24.0	40.0	36.0	3
CO6_BOVIN	Collagen alpha-1(VI) chain	57.1	42.9	0.0	2
COMP_HUMAN	Cartilage oligomeric matrix protein (COMP)	26.8	36.6	36.6	3
*Cytokines*
CTGF_PIG	Connective tissue growth factor	0.0	40.0	60.0	2
CXL16_PIG	C-X-C motif chemokine 16	0.0	0.0	100.0	1
GALA_BOVIN	Galanin message-associated peptide (GMAP)	0.0	0.0	100.0	1
IBP2_PIG	Insulin-like growth factor-binding protein 2 (IBP-2)	0.0	61.8	38.2	2
IBP5_HUMAN	Insulin-like growth factor-binding protein 5 (IBP-5)	6.8	0.0	93.2	2
IL6_BOVIN	Interleukin-6 (IL-6) [CHAIN 0]	0.0	0.0	100.0	1
*Metalloproteinases*
MMP1_HORSE	Interstitial collagenase (MMP-1)	0.0	100.0	0.0	1
MMP1_PIG	18 kDa interstitial collagenase	0.0	86.5	13.5	2
MMP2_BOVIN	PEX	0.0	0.0	100.0	1
*Inhibitors of Metalloproteinases*
TIMP1_BOVIN	Metalloproteinase inhibitor 1 (EG-1, TIMP-1)	0.0	0.0	100.0	1
TIMP1_PIG	Metalloproteinase inhibitor 1 (TIMP-1)	0.0	47.4	52.6	2
*Proteases*
HTRA1_HUMAN	Serine protease HTRA1	0.0	0.0	100.0	1
*Other*
ALBU_PIG	Serum albumin	0.0	59.6	40.4	2
PRDX1_HUMAN	Peroxiredoxin-1 (NKEF-A) (PAG)	0.0	100.0	0.0	1
PRG4_HUMAN	Proteoglycan 4 C-terminal part [ISOFORM E]	0.0	0.0	100.0	1
SAA1_MUSVI	Serum amyloid A-1 protein	0.0	100.0	0.0	1
SAA2_MUSVI	Amyloid protein A [CHAIN 0]	0.0	78.0	22.0	2
CLC11_HUMAN	C-type lectin domain family 11 member A	0.0	0.0	100.0	1

SFM = serum free medium control, CoC 1:1 = NPC: NC Co-Culture, NCCM = Conditioned Medium.
The numbers indicate peptide spectrum matches, expressed as percentage from the total over all three media. Cells were kept under normoxia.
Number in bold are percentages > 33%.

ISSN: 1471-2474