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Figure 9 | BMC Musculoskeletal Disorders

Figure 9

From: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

Figure 9

Immunological staining of brachyury (“T”, green) expression on porcine NC in alginate bead cryosections (12 μm) and DAPI stain for nuclei after 14 days of culture in presence or absence of initial pre-culture with 10% fetal calf serum (FCS) and cultured either in normoxia (~20% oxygen) or hypoxia (2% oxygen). Inlet in the upper left represents day 4 porcine NC cultured on cover slips in monolayer and stained for T. Inlet in the lower right represents a negative control lacking the primary antibody.

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