Figure 1From: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia Experimental set-up to test NC regenerative effects on co-culture with NPC or AF that were stimulated initially in pre-culture with and without 10% fetal calf serum (FCS) and subsequent co-culture or exposure with conditioned medium (NCCM) from step 1. The design also involved a comparison of normoxia (~20% oxygen) and hypoxic conditions (2% oxygen).Back to article page