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Table 1 Intra- and inter-assay precisions of PA28 γ sandwich ELISA

From: Increased proteasome activator 28 gamma (PA28γ) levels are unspecific but correlate with disease activity in rheumatoid arthritis

His-PA28γ[ng/ml]

 

0

3.125

6.25

12.5

25

50

100

200

Intra-assay CV [%], n = 3

day 1

0.4

10.4

16.7

31.2

19.9

9.3

16.8

10.4

day 2

8.7

14.7

7.6

4.6

13.8

7.9

4.9

5.2

day 3

18.1

4.9

7.0

16.5

3.9

6.5

9.1

10.9

Inter-assay CV [%], n = 3

day 1-3

10.9

10.9

10.9

18.1

15.8

11.9

14.6

13.1

  1. Precisions were calculated as the mean coefficient of variation (CV) of different PA28γ antigen concentrations measured with Sandwich ELISA in 3 independent experiments from duplicate wells at 3 days.